LOCUS       40924_5529        7964 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBAC-Lac DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Asakawa S, Abe I, Kudoh Y, Kishi N, Wang Y, Kubota R, Kudoh J, 
            Kawasaki K, Minoshima S, Shimizu N.
  TITLE     Human BAC library: construction and rapid screening
  JOURNAL   Gene 191 (1), 69-79 (1997)
  PUBMED    9210591
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Asakawa S, Shimizu N.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2001) Nobuyoshi Shimizu, Keio University School of
            Medicine, Department of Molecular Biology; 35 Shinanomachi, 
            Shinjuku-ku, Tokyo 160-8582, Japan 
            (E-mail:nshimizu@dmb.med.keio.ac.jp, Tel:81-3-5363-3755, 
            Fax:81-3-3351-2370)
REFERENCE   3  (bases 1 to 7964)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7964)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1997"; volume: "191"; issue: "1"; pages: "69-79"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2001) Nobuyoshi Shimizu, Keio University School of Medicine,
            Department of Molecular Biology"; volume: " 35 Shinanomachi, 
            Shinjuku-ku, Tokyo 160-8582, Japan 
            (E-mail:nshimizu@dmb.med.keio.ac.jp, Tel:81-3-5363-3755, Fax"; 
            pages: "81-3-3351-2370"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(67..83)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(91..107)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(115..145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(160..181)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        complement(378..395)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             complement(539..1195)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1196..1298)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      2225..2444
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2535..3287
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    3293..3543
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3869..5041
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             5044..6012
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    6088..6561
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(6821..7219)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(7237..7270)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7286..7304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7945..7961
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcgta
       61 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc
      301 ttttcaccag cgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt
      361 gcagcaagcg gtcgatccta tagtgtcacc taaatcgtat gcggccgccc gggccgtcga
      421 ccaattctca tgtttgacag cttatcatcg aatttctgcc attcatccgc ttattatcac
      481 ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg
      541 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      601 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      661 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      721 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
      781 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      841 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      901 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      961 ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     1021 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     1081 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1141 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1201 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     1261 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt
     1321 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
     1381 cttccgtcac aggtatttat tcgcgataag ctcatggagc ggcgtaaccg tcgcacagga
     1441 aggacagaga aagcgcggat ctgggaagtg acggacagaa cggtcaggac ctggattggg
     1501 gaggcggttg ccgccgctgc tgctgacggt gtgacgttct ctgttccggt cacaccacat
     1561 acgttccgcc attcctatgc gatgcacatg ctgtatgccg gtataccgct gaaagttctg
     1621 caaagcctga tgggacataa gtccatcagt tcaacggaag tctacacgaa ggtttttgcg
     1681 ctggatgtgg ctgcccggca ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg
     1741 atgctgaaac aattatcctg agaataaatg ccttggcctt tatatggaaa tgtggaactg
     1801 agtggatatg ctgtttttgt ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg
     1861 aacgaaacag tcgggaaaat ctcccattat cgtagagatc cgcattatta atctcaggag
     1921 cctgtgtagc gtttatagga agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa
     1981 cgatttgaat atgccttcag gaacaataga aatcttcgtg cggtgttacg ttgaagtgga
     2041 gcggattatg tcagcaatgg acagaacaac ctaatgaaca cagaaccatg atgtggtctg
     2101 tccttttaca gccagtagtg ctcgccgcag tcgagcgaca gggcgaagcc ctcgagtgag
     2161 cgaggaagca ccagggaaca gcacttatat attctgctta cacacgatgc ctgaaaaaac
     2221 ttcccttggg gttatccact tatccacggg gatattttta taattatttt ttttatagtt
     2281 tttagatctt cttttttaga gcgccttgta ggcctttatc catgctggtt ctagagaagg
     2341 tgttgtgaca aattgccctt tcagtgtgac aaatcaccct caaatgacag tcctgtctgt
     2401 gacaaattgc ccttaaccct gtgacaaatt gccctcagaa gaagctgttt tttcacaaag
     2461 ttatccctgc ttattgactc ttttttattt agtgtgacaa tctaaaaact tgtcacactt
     2521 cacatggatc tgtcatggcg gaaacagcgg ttatcaatca caagaaacgt aaaaatagcc
     2581 cgcgaatcgt ccagtcaaac gacctcactg aggcggcata tagtctctcc cgggatcaaa
     2641 aacgtatgct gtatctgttc gttgaccaga tcagaaaatc tgatggcacc ctacaggaac
     2701 atgacggtat ctgcgagatc catgttgcta aatatgctga aatattcgga ttgacctctg
     2761 cggaagccag taaggatata cggcaggcat tgaagagttt cgcggggaag gaagtggttt
     2821 tttatcgccc tgaagaggat gccggcgatg aaaaaggcta tgaatctttt ccttggttta
     2881 tcaaacgtgc gcacagtcca tccagagggc tttacagtgt acatatcaac ccatatctca
     2941 ttcccttctt tatcgggtta cagaaccggt ttacgcagtt tcggcttagt gaaacaaaag
     3001 aaatcaccaa tccgtatgcc atgcgtttat acgaatccct gtgtcagtat cgtaagccgg
     3061 atggctcagg catcgtctct ctgaaaatcg actggatcat agagcgttac cagctgcctc
     3121 aaagttacca gcgtatgcct gacttccgcc gccgcttcct gcaggtctgt gttaatgaga
     3181 tcaacagcag aactccaatg cgcctctcat acattgagaa aaagaaaggc cgccagacga
     3241 ctcatatcgt attttccttc cgcgatatca cttccatgac gacaggatag tctgagggtt
     3301 atctgtcaca gatttgaggg tggttcgtca catttgttct gacctactga gggtaatttg
     3361 tcacagtttt gctgtttcct tcagcctgca tggattttct catacttttt gaactgtaat
     3421 ttttaaggaa gccaaatttg agggcagttt gtcacagttg atttccttct ctttcccttc
     3481 gtcatgtgac ctgatatcgg gggttagttc gtcatcattg atgagggttg attatcacag
     3541 tttattactc tgaattggct atccgcgtgt gtacctctac ctggagtttt tcccacggtg
     3601 gatatttctt cttgcgctga gcgtaagagc tatctgacag aacagttctt ctttgcttcc
     3661 tcgccagttc gctcgctatg ctcggttaca cggctgcggc gagcgctagt gataataagt
     3721 gactgaggta tgtgctcttc ttatctcctt ttgtagtgtt gctcttattt taaacaactt
     3781 tgcggttttt tgatgacttt gcgattttgt tgttgctttg cagtaaattg caagatttaa
     3841 taaaaaaacg caaagcaatg attaaaggat gttcagaatg aaactcatgg aaacacttaa
     3901 ccagtgcata aacgctggtc atgaaatgac gaaggctatc gccattgcac agtttaatga
     3961 tgacagcccg gaagcgagga aaataacccg gcgctggaga ataggtgaag cagcggattt
     4021 agttggggtt tcttctcagg ctatcagaga tgccgagaaa gcagggcgac taccgcaccc
     4081 ggatatggaa attcgaggac gggttgagca acgtgttggt tatacaattg aacaaattaa
     4141 tcatatgcgt gatgtgtttg gtacgcgatt gcgacgtgct gaagacgtat ttccaccggt
     4201 gatcggggtt gctgcccata aaggtggcgt ttacaaaacc tcagtttctg ttcatcttgc
     4261 tcaggatctg gctctgaagg ggctacgtgt tttgctcgtg gaaggtaacg acccccaggg
     4321 aacagcctca atgtatcacg gatgggtacc agatcttcat attcatgcag aagacactct
     4381 cctgcctttc tatcttgggg aaaaggacga tgtcacttat gcaataaagc ccacttgctg
     4441 gccggggctt gacattattc cttcctgtct ggctctgcac cgtattgaaa ctgagttaat
     4501 gggcaaattt gatgaaggta aactgcccac cgatccacac ctgatgctcc gactggccat
     4561 tgaaactgtt gctcatgact atgatgtcat agttattgac agcgcgccta acctgggtat
     4621 cggcacgatt aatgtcgtat gtgctgctga tgtgctgatt gttcccacgc ctgctgagtt
     4681 gtttgactac acctccgcac tgcagttttt cgatatgctt cgtgatctgc tcaagaacgt
     4741 tgatcttaaa gggttcgagc ctgatgtacg tattttgctt accaaataca gcaatagtaa
     4801 tggctctcag tccccgtgga tggaggagca aattcgggat gcctggggaa gcatggttct
     4861 aaaaaatgtt gtacgtgaaa cggatgaagt tggtaaaggt cagatccgga tgagaactgt
     4921 ttttgaacag gccattgatc aacgctcttc aactggtgcc tggagaaatg ctctttctat
     4981 ttgggaacct gtctgcaatg aaattttcga tcgtctgatt aaaccacgct gggagattag
     5041 ataatgaagc gtgcgcctgt tattccaaaa catacgctca atactcaacc ggttgaagat
     5101 acttcgttat cgacaccagc tgccccgatg gtggattcgt taattgcgcg cgtaggagta
     5161 atggctcgcg gtaatgccat tactttgcct gtatgtggtc gggatgtgaa gtttactctt
     5221 gaagtgctcc ggggtgatag tgttgagaag acctctcggg tatggtcagg taatgaacgt
     5281 gaccaggagc tgcttactga ggacgcactg gatgatctca tcccttcttt tctactgact
     5341 ggtcaacaga caccggcgtt cggtcgaaga gtatctggtg tcatagaaat tgccgatggg
     5401 agtcgccgtc gtaaagctgc tgcacttacc gaaagtgatt atcgtgttct ggttggcgag
     5461 ctggatgatg agcagatggc tgcattatcc agattgggta acgattatcg cccaacaagt
     5521 gcttatgaac gtggtcagcg ttatgcaagc cgattgcaga atgaatttgc tggaaatatt
     5581 tctgcgctgg ctgatgcgga aaatatttca cgtaagatta ttacccgctg tatcaacacc
     5641 gccaaattgc ctaaatcagt tgttgctctt ttttctcacc ccggtgaact atctgcccgg
     5701 tcaggtgatg cacttcaaaa agcctttaca gataaagagg aattacttaa gcagcaggca
     5761 tctaaccttc atgagcagaa aaaagctggg gtgatatttg aagctgaaga agttatcact
     5821 cttttaactt ctgtgcttaa aacgtcatct gcatcaagaa ctagtttaag ctcacgacat
     5881 cagtttgctc ctggagcgac agtattgtat aagggcgata aaatggtgct taacctggac
     5941 aggtctcgtg ttccaactga gtgtatagag aaaattgagg ccattcttaa ggaacttgaa
     6001 aagccagcac cctgatgcga ccacgtttta gtctacgttt atctgtcttt acttaatgtc
     6061 ctttgttaca ggccagaaag cataactggc ctgaatattc tctctgggcc cactgttcca
     6121 cttgtatcgt cggtctgata atcagactgg gaccacggtc ccactcgtat cgtcggtctg
     6181 attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc
     6241 acggtcccac tcgtatcgtc ggtctgataa tcagactggg accacggtcc cactcgtatc
     6301 gtcggtctga ttattagtct gggaccatgg tcccactcgt atcgtcggtc tgattattag
     6361 tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggaa ccacggtccc
     6421 actcgtatcg tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct
     6481 gattattagt ctgggaccac gatcccactc gtgttgtcgg tctgattatc ggtctgggac
     6541 cacggtccca cttgtattgt cgatcagact atcagcgtga gactacgatt ccatcaatgc
     6601 ctgtcaaggg caagtattga catgtcgtcg taacctgtag aacggagtaa cctcggtgtg
     6661 cggttgtatg cctgctgtgg attgctgctg tgtcctgctt atccacaaca ttttgcgcac
     6721 ggttatgtgg acaaaatacc tggttaccca ggccgtgccg gcacgttaac cgggctgcat
     6781 ccgatgcaag tgtgtcgctg tcgacgagct cgcgagctcg gacatgaggt tgccccgtat
     6841 tcagtgtcgc tgatttgtat tgtctgaagt tgtttttacg ttaagttgat gcagatcaat
     6901 taatacgata cctgcgtcat aattgattat ttgacgtggt ttgatggcct ccacgcacgt
     6961 tgtgatatgt agatgataat cattatcact ttacgggtcc tttccggtga tccgacaggt
     7021 tacggggcgg cgacctcgcg ggttttcgct atttatgaaa attttccggt ttaaggcgtt
     7081 tccgttcttc ttcgtcataa cttaatgttt ttatttaaaa taccctctga aaagaaagga
     7141 aacgacaggt gctgaaagcg agctttttgg cctctgtcgt ttcctttctc tgtttttgtc
     7201 cgtggaatga acaatggaag tccgagctca tcgctaataa cttcgtatag catacattat
     7261 acgaagttat attcgatgcg gccgctaata cgactcacta tagggagaag ctgatctaca
     7321 aaggctatca ggtcattgcc tgagagtctg gagcaaacaa gagaatcgat gaacggtaat
     7381 cgtaaaacta gcatgtcaat catatgtacc ccggttgata atcagaaaag ccccaaaaac
     7441 aggaagattg tataagcaaa tatttaaatt gtaaacgtta atattttgtt aaaattcgcg
     7501 ttaaattttt gttaaatcag ctcatttttt aaccaatagg aacgccatca aaaataattc
     7561 gcgtctggcc ttcctgtagc cagctttcat caacattaaa tgtgagcgag taacaacccg
     7621 tcggattctc cgtgggaaca aacggcggat tgaccgtaat gggataggtt acgttggtgt
     7681 agatgggcgc atcgtaaccg tgcatctgcc agtttgaggg gacgacgacc gtatcggcct
     7741 caggaagatc gcactccagc cagctttccg gcaccgcttc tggtgccgga aaccaggcaa
     7801 agcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     7861 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     7921 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgcc
//
